Facility: Proteomics and Functional Genomics Core Facility

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Function: Analytical/Synthesis

Department: Mathematical and Natural Sciences
College/Unit: New College of Interdiciplinary Arts & Sciences

Description:

Faculty in ASU West's Division of Mathematical and Natural Sciences have research interests that range from the study of single molecules to the ecology of organisms indigenous to arid regions. To gain an unprecedented level of insight into each of the organisms and systems they study, several faculty employ tools in proteomics and functional genomics. Mathematical biologists in the Division collaborate with life and natural scientists in the Division to make sense of the masses of data that typically result from experiments utilizing proteomics and functional genomics-based tools.

Link to More Details:
http://newcollege.asu.edu/mns/facilities/proteomics

Facility People

Resources (7)

Axon GenePix 4100A Microarray Scanner

For microarray data acquisition and analysis, our Core Facility contains an Axon GenePix 4100A microarray scanner. GenePix software to facilitate data acquisition and analysis is also available.

Bio-Rad Versadoc 400 Gel Imaging System

Capable of imagaging both nucleic acid and protein gels.

Bruker Biospin AVANCETM III 400 NMR

The Bruker Biospin AVANCETM III 400 Spectrometer System utilizes a superconducting magnet system with 5.4 cm bore (operation field at 9.395 Tesla). The unit is equipped with a sample transport system that enables high flow rates for VT gas. Bruker TopSpin software is used for data acquisition and processing.

Matrix-Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF) Mass Spectrometer

For routine protein characterization and identification, our Core Facility contains a Matrix-Assisted Laser Desorption Ionization TIme-of-Flight (MALDI-TOF) mass spectrometer (MS). Specifically, the unit is a Bruker Microflex LRF equipped with a 60 Hz laser and is capable of measuring masses of molecules ranging in size from several hundred to several hundred thousand Daltons. A comprehensive suite of software is available to assist in collecting, analyzing, interpreting, and presenting data.

Protein Separation and Preparation Equipment

A variety of tools are available to assist with protein separation and preparation including a Bio-Rad LP protein purification system with fraction collector, benchtop centrifuges, standard and ultracentrifuges. A rotovap system is available to concentrate samples.

Spectrophotometers

The Core Facility contains a host of spectrophotometers and a plate reader to characterize and quantify proteins, nucleic acids, and other biological molecules. In addition, a nanodrop spectrophotometer is available.

Thermal Cycler

The Core Facility contains two thermal cyclers and an MJ Opticon real-time thermal cycler.

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