Facility: Microscale Thermophoresis Facility

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Function: Analytical/Synthesis

Department: School of Molecular Sciences (SOMS)
College/Unit: COLLEGE OF LIBERAL ARTS AND SCIENCES

Description:

MicroScale Thermophoresis (MST) is an easy, fast and precise way to quantify biomolecular interactions. It measures the motion of molecules along microscopic temperature gradients and detects changes in their hydration shell, charge or size. When performing a MST experiment, a microscopic temperature gradient is induced by an infrared laser, and the directed movement of molecules is detected and quantified using either covalently attached dyes, or fluorescent fusion protein. MST detects interactions between any kind of biomolecules thus providing a large application range, from ions and small molecules to high molecular weight and multi-protein complexes.

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Microscale Thermophoresis (MST)

Thumb MicroScale Thermophoresis (MST) is an easy, fast and precise way to quantify biomolecular interactions. It measures the motion of molecules along microscopic temperature gradients and detects changes in their hydration shell, charge or size. When performing a MST experiment, a microscopic temperature gradient is induced by an infrared laser, and the directed movement of molecules is detected and quantified using either covalently attached dyes, or fluorescent fusion protein. MST detects interactions between any kind of biomolecules thus providing a large application range, from ions and small molecules to high molecular weight and multi-protein complexes. Current instrumentation is a Monolith NT.115 Series unit by NanoTemper Technologies. For access and assistance, please contact Dr. Julian Chen (JLChen@asu.edu, 480-965-3650) or Patrick Gleason (prgleaso@asu.edu, 480-965-1928)

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