Facility: CLAS DNA Laboratory

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Function: Analytical/Synthesis

Department: CLAS Research Technical Services


The DNA Laboratory is managed by Scott Bingham on the main campus of Arizona State University. The location is in Life Sciences E-wing room S-17.  It offers an array of technical and support services. DNA Sequencing and Fragment Analysis Service are supported by an Applied Biosystems (ABI) 3730 capillary sequencer. Facilities also include an Agilent Microarray Scanner and an ABI Real Time PCR system, Agilent Bioanalyzer, and Biotek Plate Reader.   Click HERE to register for laboratory services. To schedule equipment or to submit samples for analysis, registered users please navigate directly to the ASU DNA Core Lab

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96 Well Microplate Reader

Thumb 96 Well Plate Reader: The Biotek HT1 Plate Reader scans multiple plate types in Absorbance, Fluorescence or Luminescence modes. Training is provided on the software and plate setup. The HT1 is equipped with gas control to allow anaerobic measurements or measurements under other environmental conditions. DNA and protein quantitation can also be done in a small volume 16 well plate. Reservations are taken for daily use.

Bioanalyzer (Agilent 2100)

Thumb Bioanalyzer: The Agilent 2100 Bioanalyzer is a capillary electrophoresis unit capable of separating RNA, DNA or protein in lieu of gel electrophoresis. You may submit samples as a service request or you can be trained how to use the instrument. We routinely run RNA and DNA samples only, so please contact us before planning to submit any other samples. Chip types usually on hand are DNA1000, High Sensitivity DNA, RNA6000 Nano and RNA6000 Pico. Reservations are taken for daily use.

DNA Sequencing and Fragment Analysis

Thumb DNA sequencing is run as a service. Laboratories may submit samples along with the sequencing request form. Sequencing reactions are usually run in our lab. However, we will run, at reduced cost, sequencing reactions — run in other laboratories. We will purify the reactions before running. We maintain a stock of common plasmid primers accessible on the sequencing form. We use an Applied Biosystems 3730 capillary sequencer. Read lengths are routinely 800–900bp. Turnaround time is 24–48 hours. We can also perform plasmid or pcr purifications in conjunction with sequencing. Fragment Analysis, which includes microsatellites, AFLP, or STR applications, are run on the 3730 sequencer. Dye-labeled samples are normally generated in respective laboratories and delivered to our lab for analysis. Contact us if you have questions on dye compatibility and before submitting samples.

DNA Size Fractionation

Thumb DNA Size Fractionation: Preparative DNA size fractionation can be run on a Sage Scientific Pippen Prep. Recovered samples are suitable for Next-Gen sequencing. There can be up to four samples per run.

Microarray Analysis

Thumb Microarray analysis: Slides can be scanned using an Agilent scanner. We do not manufacture microarrays. We do have the equipment to complete all other aspects of microarray analysis. Files generated from the scanner are processed using either Agilent’s Feature Extraction or Axon’s GenePix Pro 6.0 software.. Normally we instruct users on the techniques and software. However, we will provide microarray hybridization and analysis to laboratories that provide us the RNA. Contact us for more information. Any RNA submitted for microarray analysis will be checked on the bioanalyzer for integrity before beginning the labeling reactions.

Real-Time PCR (ABI 7900)

Thumb Real time PCR in the DNA lab is run on an ABI7900HT thermocycler using a 384 well format. Samples are usually set up in respective laboratories. We provide training on the software and help in experimental setup, if required. Primers and probes can be designed using ABI Primer Express software available in the lab. Reservations are taken for three daily time slots.

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