Facility: CLAS Bioimaging Facility - Keck Division

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Function: Analytical/Synthesis

Department: CLAS Research Technical Services


The W. M. Keck division of the CLAS Bioimaging Facility supports a broad range of individual and collaborative studies by over 150 scientists in the southwestern United States. It is managed by Page Baluch and Douglas Chandler. The laboratory serves as a catalyst to consolidate ASUs bioimaging capabilities, to launch the development of new bioimaging curricula for ASU students, and to strengthen university relationships with external partners such as Barrow Neurological Institute, Mayo Clinic Scottsdale, and the NIH Diabetes Facility in Phoenix. The W. M. Keck laboratories house some of the latest bioimaging technology including, laser scanning confocal microscopy; video light microscopy; fluorescence microscopy; live cell microscopy; multi-photon microscopy, and image processing.

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Biosafety Protocols for Live Cell Imaging

Thumb Live sample imaging is becoming a routine method of imaging cells but require that additional precautions be established to ensure a safe environment within a shared facility. This link contains the guidelines established by the Arizona State University EHS and Keck Bioimaging Laboratory. These define the cell lines and organisms permitted in the Keck lab and the procedures for cleanup and disposal of contaminated materials.

Evos FL Auto Live Cell Imaging System

Thumb The Evos inverted microscope system is equipped with an onstage incubator for environmental control and can accommodate multi-well plates and standard round culture dishes. The fluorescent/widefield system is capable of z stage movement and tiling. Phase corrected objectives include 4xPH, 10xPH, 20xL PH, 40xL PH as well as a 60x 1.42NA oil lens for higher resolution imaging. Four LED light cubes are available to cover the standard DAPI, GFP, RFP and Cy5 wavelengths.

Leica CM1950 Cryostat

Thumb Leica CM1950 cryostat has a precise microtome object feed mechanism for high-quality sections, CryoZone™ system for optimal sectioning conditions and UVC disinfection and AgProtect™ for operator safety.

Leica RM2235 Microtome

Thumb Designed for manual routine paraffin embedded tissue sectioning.

Leica SP2 Confocal Microsope

Thumb The Leica TCS SP2 AOBS Laser Scanning Confocal system is designed for confocal imaging and quantitative measurement using laser point scanning of fluorescence labeled living and fixed specimens. Multiple laser excitation lines provide the system with the ability to excite almost any fluorophore including tunable emission filter capability. The tunable AOBS beam splitter allows simultaneous 3 channel acquisition. The Leica software can perform multi-dimensional image processing in both xyz and xzy, FRET, FRAP, and lambda scanning.

Leica TCS SP5 AOBS Spectral Confocal System

Thumb The Leica SP5 has four laser lines (405, 488 [458, 476, 496,514], 561 and 633) and is capable of capturing 5 channels which includes a DIC bright field detector. The microscope is an upright DM6000 and has a motorized stage. Other features include; pixel resolution up to 8000x8000, AOTF laser modulation, 200 degree field rotation, standard objective set plus a 20x multi-emersion objective, electrophysiology software package and a Bioptics environmental chamber unit.

Nikon Elipse TE300 Inverted Video Microscope

Thumb This imaging system consists of a Nikon Elipse TE300 inverted microscope and is outfitted with two cameras; a Hamamatsu Orca 3CCD and a Olympus DP26 color camera that allows real-time digital image capture. Both fluorescent and visible light (including both Phase and DIC contrast enhancement capabilities) are supported.

Nikon Microscope w/ Eppendorf Microinjection

Thumb Nikon microscope with Eppendorf microinjection workstation

Prairie Ultima IV 2-Photon Microscope

Thumb The Prairie Ultima IV 2P microscope comes equipped with an Ultima IV in vivo laser scanning microscope with an X-Y translation stage. The dual laser system includes the Mai Tai and Tsunami lasers capable of MPE imaging, oblation and uncaging.

Technical Assistance

Thumb Training to use the instruments within the Keck Bioimaging Laboratory is free of charge. A technical assistance fee is charged for any work or imaging that is done as a service for a lab.

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