Leica SP2 Confocal Microsope

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Managed By: CLAS Bioimaging Facility - Keck Division

Description:
The Leica TCS SP2 AOBS Laser Scanning Confocal system is designed for confocal imaging and quantitative measurement using laser point scanning of fluorescence labeled living and fixed specimens. Multiple laser excitation lines provide the system with the ability to excite almost any fluorophore including tunable emission filter capability. The tunable AOBS beam splitter allows simultaneous 3 channel acquisition. The Leica software can perform multi-dimensional image processing in both xyz and xzy, FRET, FRAP, and lambda scanning.


For More information on this resource, visit: http://leica-microsystems.com

Resource Manager Email: | Phone: 480-727-0725

Location: LS - Life Sciences Center , C-Wing L2-74

Resource Type: Equipment

Schedule this resource online: asu.corefacilities.org

Hours of Operation: 24 Hour Scheduling, Monday-Friday

Technical Specifications

  • TCS SP2 AOBS Spectral Confocal Scanner mounted on a Leica DMRE upright fluorescent microscope
  • 3 lasers (Ar, Kr/Ar, and He/Ne)
  • 3 prism spectrophotometer detectors
  • 458, 476, 488, 514, 568, and 633nm laser excitation lines
  • Adjustable emissions bandwidth for customized collection of fluorescence
  • 5X, 10X and 20X dry, 40X and 100X oil and 63X water immersion objectives
  • 20X, 40X and 63x dipping lenses
  • Leica Confocal Software (LCS) for multi-dimensional image series acquisition, image processing and analysis, 3D reconstruction and measurement, FRET, FRAP, Lambda Scanning, dye separation , multicolor restoration

Applications

Some useful applications of a confocal system include but not limited to: acquisition of high quality fluorescence images of a single optical slice, acquisition of images from thick section s requiring severe background suppression, reconstruction of 3-D images, co-localization and expression studies,  as well as kinetic studies such as FRET or FRAP.

Categories

Analytical and Testing - Bio-Molecular/Cellular Analytical and Testing - Imaging and Microscopy


Online References (Links):

Leica Microsystems (http://www.leica-microsystems.com/products/confocal-microscopes/)

Additional References (Files):

Keyword(s):

Microscope, confocal, LSCM, fluorescence, SP2