Leica TCS SP5 AOBS Spectral Confocal System

Home > CLAS Bioimaging Facility - Keck Division >Leica TCS SP5 AOBS Spectral Confocal System

Managed By: CLAS Bioimaging Facility - Keck Division

The Leica SP5 has four laser lines (405, 488 [458, 476, 496,514], 561 and 633) and is capable of capturing 5 channels which includes a DIC bright field detector. The microscope is an upright DM6000 and has a motorized stage. Other features include; pixel resolution up to 8000x8000, AOTF laser modulation, 200 degree field rotation, standard objective set plus a 20x multi-emersion objective, electrophysiology software package and a Bioptics environmental chamber unit.

For More information on this resource, visit: http://leica-microsystems.com

Resource Manager Email: | Phone: 480-727-0725

Location: LS - Life Sciences Center , LSC L2-80a

Resource Type: Equipment

Schedule this resource online: asu.corefacilities.org

Hours of Operation: 24 Hour Scheduling, All 7 Days

Technical Specifications

  • TCS SP5 AOBS Spectral Confocal Scanner mounted on a Leica DM6000 upright fluorescent microscope
  • 4 lasers (405, Ar, Kr/Ar, and He/Ne)
  • 4 prism spectrophotometer detectors
  • 405, 458, 476, 488, 514, 561, and 633nm laser excitation lines
  • Adjustable emissions bandwidth for customized collection of fluorescence
  • 5X, 10X and 20X dry, 20x (oil, water, glycerol), 40X, 63X and 100X oil and 63X water immersion objectives
  • 20X, 40X and 63x dipping lenses
  • Galvanometer precision z-stage capable of mapping and montage image capture
  • Transmitted light detector with automated DIC optics
  • Leica Confocal Software (LCS) for multi-dimensional image series acquisition, image processing and analysis, 3D reconstruction and measurement, FRET, FRAP, Lambda Scanning, dye separation , multicolor restoration


Some useful applications of a confocal system include but not limited to: acquisition of high quality fluorescence images of a single optical slice, acquisition of images from thick section s requiring severe background suppression, reconstruction of 3-D images, co-localization and expression studies,  as well as kinetic studies such as FRET or FRAP.


Analytical and Testing - Bio-Molecular/Cellular Analytical and Testing - Imaging and Microscopy Engineering and Applied Science Sciences - Life and Environmental

Additional References (Files):


Microscope, confocal, LSCM, fluorescence