Biosafety Protocols for Live Cell Imaging

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Managed By: CLAS Bioimaging Facility - Keck Division

Description:
Live sample imaging is becoming a routine method of imaging cells but require that additional precautions be established to ensure a safe environment within a shared facility. This link contains the guidelines established by the Arizona State University EHS and Keck Bioimaging Laboratory. These define the cell lines and organisms permitted in the Keck lab and the procedures for cleanup and disposal of contaminated materials.


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Location: LS - Life Sciences Center , LSC L2-80e

Resource Type: Procedure

Live sample imaging is becoming a routine method of imaging cells but require that additional precautions be established to ensure a safe environment within a shared facility. Below are the guidelines established by the Arizona State University EHS and Keck Bioimaging Laboratory. These define the cell lines and organisms permitted in the Keck lab and the procedures for cleanup and disposal of contaminated materials. For additional guidelines in working with biohazardous materials see the Standard Operating Procedure as defined by the CDC.  If you have any questions about this protocol please contact the lab manager (Page Baluch, page.baluch@asu.edu: 480-727-0725)

Cell lines and organisms allowed for imaging within the Keck Bioimaging Lab:

  • BSL1 – Animal derived cell lines and organisms such as yeast or E coli are under this category.
  • BSL2 - All human derived cells (i.e. HeLa or Neuroblastoma) which may contain pathogens (i.e. Human Papilloma virus)

NOT allowed in the Keck Bioimaging lab:

  • BSL3&4 – Include microorganisms in which there is no cure and will cause severe illness or death such as Tuberculosis or Ebola. BSL3 & 4 organisms are NOT permitted in the Keck Bioimaging Laboratory.

Standard operating procedure for BSL2 samples transported to the Keck Bioimaging Lab

The Keck lab has PPE equipment available in LSC L2-80e (lab jackets, gloves, safety glasses) as well as a BSL2 certified cell culture hood and incubator that can be used to stabilize your cells.There are also hazardous waste containers for media and contaminated materials (including sharps).

1. Samples should be prepared in your lab using the appropriate BSL hoods and safety procedures. Culture dishes must be wrapped in parafilm and placed in a primary, non-leaking container (i.e. Ziploc bag or plastic box) then placed in a secondary container with absorbent napkins at the base.

2. After arriving to the Keck lab, wash your hands and then put on one of the available lab jackets, gloves and safety glasses.

3. The dishes should be removed from the primary and secondary containers and then wiped with Kim wipes that are damp with 70% ethanol before placing into the incubator or onto the microscope stage.

4. Once your dishes have been placed onto the microscope stage, remove gloves before touching the microscope or computer.

5. After imaging, the samples must be removed from the microscope and the microscope stage and any bench space used should be wiped down with Kim wipes that are damp with 10% bleach followed by 70% ethanol. Any spills must be cleaned immediately with bleach followed with ethanol. Please inform the Keck lab staff if you do spill something so we can ensure that the microscope is thoroughly cleaned following your imaging session.

6. The Keck Lab has biosafety waste containers available in LSC L2-80e and L2-66a for media and materials waste.

7. Return the lab jacket and safety glasses and wash your hands with soap before you leave the lab.

Categories

Analytical and Testing - Imaging and Microscopy Sciences - Life and Environmental Specialized Rooms, Spaces, Facilities


Online References (Links):

CDC Biosafety Level Laboratory Guidelines (http://www.cdc.gov/biosafety/publications/bmbl5/BMBL5_sect_IV.pdf)

Additional References (Files):

Keyword(s):

bioimaging, live-cell, biosafety