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96 Well Microplate Reader, Facility:CLAS DNA Laboratory

Thumb 96 Well Plate Reader: The Biotek HT1 Plate Reader scans multiple plate types in Absorbance, Fluorescence or Luminescence modes. Training is provided on the software and plate setup. The HT1 is equipped with gas control to allow anaerobic measurements or measurements under other environmental conditions. DNA and protein quantitation can also be done in a small volume 16 well plate. Reservations are taken for daily use.

Agilent 7890 Automated Gas Analyzer, Facility:CLAS Goldwater Environmental Laboratory

Thumb This gas chromatography (GC) analyzer is set up to quantify three common greenhouse gases (GHGs) simultaneously.The analysis of air and headspace gas content is useful for environmental monitoring, soil gas flux, catalysis research, closed chamber respiration studies, and beyond. The flame ionization detector (FID) signals for methane (CH₄) and the micro-cell electron capture detector (µ-ECD) will detect nitrous oxide (N₂O) content. A 3rd Li-820 detector for carbon dioxide (CO₂) directly measures CO₂. Automated injections allows for swift and simultaneous analysis. Other analytes such as sulfur hexafluoride (SF₆) may also be analyzed. Please enquire for more information.

Axon GenePix 4100A Microarray Scanner, Facility:Proteomics and Functional Genomics Core Facility

For microarray data acquisition and analysis, our Core Facility contains an Axon GenePix 4100A microarray scanner. GenePix software to facilitate data acquisition and analysis is also available.

BET Surface Area and Porosity Analysis - Tristar II 3020, Facility:CLAS Goldwater Environmental Laboratory

Thumb The Tristar II 3020 provides high-quality surface area and porosity measurements on solid materials by using the technique of gas adsorption. The TriStar II is a fully automated, three-station analyzer capable of increasing the speed and efficiency of quality control analyses, with the accuracy, resolution, and data reduction to meet most research needs. SEE MATERIAL REQUIREMENTS BELOW

Ball Mill - Spex-Certiprep 8000D, Facility:CLAS Goldwater Environmental Laboratory

Thumb Up to 14 samples can be processed simultaneously with dual rotars. Stainless steel vials and balls are available for grinding rocks, sediments, and other hard materials that resist standard teqhniques.

Bio-Rad Versadoc 400 Gel Imaging System, Facility:Proteomics and Functional Genomics Core Facility

Capable of imagaging both nucleic acid and protein gels.

Biosafety Protocols for Live Cell Imaging, Facility:CLAS Bioimaging Facility - Keck Division

Thumb Live sample imaging is becoming a routine method of imaging cells but require that additional precautions be established to ensure a safe environment within a shared facility. This link contains the guidelines established by the Arizona State University EHS and Keck Bioimaging Laboratory. These define the cell lines and organisms permitted in the Keck lab and the procedures for cleanup and disposal of contaminated materials.

Bruker Avance III HD 600 MHz NMR, Facility:CLAS Magnetic Resonance Research Center

Thumb This Bruker 600 MHz NMR is equipped with a 5 mmTriple Resonance Prodigy Cryoprobe. CPP TCI 600 H&F-C/N-D-5-Z. It is ideally suited for biological and protein structure determination. Additionally protein and bimolecular dynamics in solution can be studied with this instrument.

Bruker Avance III HD 850 MHz NMR, Facility:CLAS Magnetic Resonance Research Center

Thumb The Bruker 850 MHz spectrometer is equipped with a 5 mm H&F-C/N-D TCI Cryoprobe, a 1.7 mm H-C/N-D TCI Cryoprobe, and 1.3 mm Ultrafast HX MAS probe. This instrument is equipped for liquids and solids. The instrument is ideally suited for the study of complex biomolecules.

Bruker Biospin AVANCETM III 400 NMR, Facility:Proteomics and Functional Genomics Core Facility

The Bruker Biospin AVANCETM III 400 Spectrometer System utilizes a superconducting magnet system with 5.4 cm bore (operation field at 9.395 Tesla). The unit is equipped with a sample transport system that enables high flow rates for VT gas. Bruker TopSpin software is used for data acquisition and processing.

CHN Elemental Analysis - PE2400, Facility:CLAS Goldwater Environmental Laboratory

Thumb The PE 2400 is a conventional elemental analyzer. It operates by flash combustion of the specimen encapsulated in tin cups or disks whereby the specimen is completely combusted at a temperature of 1760 C. The resulting gases are chemically scrubbed of the halogens (and of sulfur in the CHN mode) and are separated in a GC column. Detection is conducted by a thermal conductivity detector (TCD). Total C, H, and N are calculated with a precision of 0.1g. Element ratios are more significant since they minimize instrument error. Molar element ratios may be reliably reported for up to three decimal places.

Carbon Isotope Analyzer - Picarro, Facility:CLAS Goldwater Environmental Laboratory

Thumb The PICARRO G2201-i CRDS Analyzer is capable of simultaneous measurement of carbon isotopes from both CO2 and CH4. The G2201-i operates in one of three modes: CO2-only mode, CH4-only mode, and combined CO2/CH4 mode. The Small Sample Isotope Module 2 (SSIM2) expands the measurements that can be made with the Picarro δ13C analyzers for CO2 and CH4. This allows user to: 1. Run gas samples as small as 20 ml at ambient CO2 or CH4 concentrations, 2. Measure microliter volumes of high-concentration samples with built-in dilution capability. 3.Inject samples by syringe

Centrifuge (Allegra X-14 Benchtop) , Facility:CLAS Goldwater Environmental Laboratory

Thumb Centrifuge has both swinging bucket rotor (max. 4300 rpm) and fixed angle rotor (max. 10,200 rpm) capabilities. Rotors have adaptors for both 15 ml and 50 ml conical vials. Centrifuge has capability for both timed or user programed options.

Cutting Mill - Wiley, Facility:CLAS Goldwater Environmental Laboratory

Useful for cutting/grinding fibrous materials such as plant stems, leaves, and roots that do not lend themselves well to ball-mill grinding. Assorted mesh sizes are available to control particle size.

DNA Size Fractionation, Facility:CLAS DNA Laboratory

Thumb DNA Size Fractionation: Preparative DNA size fractionation can be run on a Sage Scientific Pippen Prep. Recovered samples are suitable for Next-Gen sequencing. There can be up to four samples per run.

Digital Repository Services, Facility:ASU Digital Repository

ASU Digital Repository is an open access repository that will provide long-term, reliable storage and access to ASU related research. The ASU-DR stores and makes available scholarly work that is complete and ready for dissemination, such as articles, working papers, technical reports, presentations, recordings of performances, images, data sets, and more. The ASU-DR helps meet public access policies and archival requirements specified by many federal grants by providing long-term preservation for materials. Most uses of the ASU Digital Repository are provided at no charge to the ASU community.

Discrete Analyzer - Seal AQ2, Facility:CLAS Goldwater Environmental Laboratory

Thumb The AQ2 system is a flexible automated analyzer that uses the principle of Discrete Analysis where each test occurs in a separate or discrete reaction vessel. The AQ2 is ideal for measuring dissolved nutrients and other constituents in aqueous samples, extracts, and digestions. It is most useful when many and varied tests are needed on different samples and/or individual results are needed immediately. The sample and reagents are incubated in the reaction wells for a pre-programmed time. A single aliquot is then transferred into a 10mm 100% optical glass cuvette. The absorbance is read on the stationary reactant to ensure the best possible signal to noise ratio. Methods: Alkalinity Ammonia Chloride Cyanides Nitrate/Nitrite Nitrite Nitrogen, Total (Persulfate) Phenolics Phosphate, Ortho Phosphorus, Total (Persulfate) Silicate Sulfate

Electronics Services, Facility:Instrument Design and Fabrication Core

Services include:Laboratory Assistance: Install laboratory electronic equipment and assist with setting up research and teaching. Labs, including design and fabrication of test modules. Provide purchase recommendations for laboratory equipment, parts and supplies. Assist with lab safety issues including design and installation of door interlocks warning signs etc. Electronic equipment repair: Provide low-level repair support for analytical laboratory equipment. (Gas chromatographs, Spectrophotometers, etc.) Repair general purpose test equipment. (oscilloscopes, digital multimeters, function generators, etc.). Labview: Install and upgrade older versions of Labview in Academic labs. Install associated hardware and provide purchasing advice for A/D boards and interface modules. Design & Fabrication: We can assist faculty and graduate students with electronic design and once the design is finalized we can do the fabrication. Ensure equipment and test setups comply with safety requirements. Training: User training for operating general-purpose test equipment.

Evos FL Auto Live Cell Imaging System, Facility:CLAS Bioimaging Facility - Keck Division

Thumb The Evos inverted microscope system is equipped with an onstage incubator for environmental control and can accommodate multi-well plates and standard round culture dishes. The fluorescent/widefield system is capable of z stage movement and tiling. Phase corrected objectives include 4xPH, 10xPH, 20xL PH, 40xL PH as well as a 60x 1.42NA oil lens for higher resolution imaging. Four LED light cubes are available to cover the standard DAPI, GFP, RFP and Cy5 wavelengths.

Flow Injection Analysis - Lachat QC8000, Facility:CLAS Goldwater Environmental Laboratory

Thumb The QC 8000 determines analyte concentrations using colorimetry to detect changes in color produced by the presence of analytes. It is typically used for automated testing of water, waste water and sea water. It is capable of measuring a wide range of analytes, given the appropriate manifold configuration and filter. Currently we are able to measure: Ammonia, Chloride, Nitrate, Nitrite, Total Nitrogen (using an in-line digestion), Orthophosphate, Total Phosphorus (using an in-line digestion), and Alkalinity (methyl orange as a function of CaCO3). It can be expanded to measure dozens of other analytes as well. The instrument is equipped with a 360 position autosampler for multiple sample analysis and an autodilutor for dilution of off-scale samples. It is capable of processing a combination of two analytes simultaneously.

Furnace Atomic Absorbance Spectrometry - Varian 400 , Facility:CLAS Goldwater Environmental Laboratory

Thumb With this instrument, user can expect detection limits lower than can be achieved by either flame AA or ICP (usually sub-ppb, but element specific) without the high cost associated with ICP-MS. In addition to making multiple sample analysis less burdensome, the attached auto sampler aids in increasing precision, adding any modifiers necessary, and may be used to generate/regenerate and check standard curves. The instrument is equipped with Zeeman background correction capabilities.

GEARS An Infrastructure for Energy-Efficient Big Data Research on Heterogeneous and Dynamic Data, Facility:CASCADE - Center for Assured and Scalable Data Engineering

GEARS (an enerGy-Efficient big-datA Research System) is a heterogeneous computing and storage resource based on cohesively co-designed software and hardware components. It enables a variety of important studies on heterogeneous and dynamic data and advances the scientific knowledge in data-driven disciplines. The hardware of GEARS includes a cluster of data nodes equipped with heterogeneous processors and storage devices and fine-grained power management capability. The software is developed upon widely-used big data frameworks to support unified programming across CPUs, GPUs, and FPGAs, and transparent data access across a deep storage hierarchy integrating DRAM, NVM, SSD, and HDD.

Gas Chromatography / MS/MS - Varian Ion Trap , Facility:CLAS Goldwater Environmental Laboratory

Thumb The Saturn is used for both qualitative (identification/confirmation) and quantitative determinations of trace levels of organic compounds and is capable of both electron ionization (EI) and chemical ionization (CI) using liquid reagents in either MS or MS/MS modes. Selective Ion Storage (SIS) can be used to isolate one or more specific ions in the presence of a much higher background. MS/MS (tandem MS) enables selective storage and subsequent fragmentation of a precursor ion and can be used to further eliminate background and/or to differentiate analytes with very similar GCMS spectra. The NIST 2003 library is available for spectral searches.

Gas Chromatography / Mass Spectrometry - HP Quadrupole, Facility:CLAS Goldwater Environmental Laboratory

Thumb Comprised of an HP 5890 GC interfaced to a HP 5972 Mass Selective Detector Quadrupole Mass Spectrometer, the system uses electron impact (EI) ionization to produce mass fragmentation and is capable of performing full mass scans or selective ion monitoring (SIM) of one or more specific masses. The system is equipped with one split/splitless capillary injection port which will accommodate liquid, headspace, or SPME injections.

HPLC Analysis, Facility:Lipid Soluble Nutrients Lab

use of reverse-phase high performance liquid chromatography to determine types and amounts of carotenoids in plant or animal tissue

ICP Optical Emission Spectrometer - Themo iCAP6300, Facility:CLAS Goldwater Environmental Laboratory

Thumb The iCAP is capable of rapid, simultaneous measurement of up to 70 elements in aqueous and organic solutions and is equipped with an autosampler with a 240 sample capacity. The dual view design offers the flexibility of axial view for lowest detection limits and radial view for reduced interference; ideal for complex samples often encountered in environmental research. Full frame imaging produces a picture of a sample and can be a useful qualitative tool in determining sample composition. Detection limits vary by element but are typically in the low to sub-ppb (ug/L) level.

Ion Chromatography - Dionex ICS 2000, Facility:CLAS Goldwater Environmental Laboratory

Thumb The ICS 2000 instrument is configured as a 2 mm system for high-sensitivity (sub-ppb) analysis. It is equipped with a potassium hydroxide eluent generator capable of gradient or stepped analysis and a column heater. These features offer improved peak resolution and sensitivity compared to the ICS 1000 system. Sample filtration via a 0.45 micron pore size filter (or smaller) is required.

Ion Chromatography - Dionex ICS1000, Facility:CLAS Goldwater Environmental Laboratory

Thumb This IC system determines analyte concentrations using chromatography columns and a pulsed electrochemical conductivity detector. It is typically used for automated testing of aqueous solutions. It is capable of measuring a wide variety of ions, given the appropriate eluant and analytical column. Currently, we are able to measure the major anions: Fluoride, Chloride, Bromide, Nitrate, Nitrite, Phosphate, and Sulfate as well as numerous other anions using an AS23 column. It can be reconfigured to analyze cations if required. The autosampler will accommodate up to 66 vials of 5 mL size, 88 vials of 0.5 mL size, or a combination of the two sizes.

KODAK Image Station In-Vivo FX, Facility:CLAS Bioimaging Facility - Keck Division

Thumb The In-Vivo FX offers quantitative imaging of luminescent, radioisotopic and fluorescent labeled biomolecules in combination with radiographic imaging, providing a versatile in vivo imaging system for small animals. It also has the ability to select and apply longer excitation wavelengths (green to near-IR) which improves the penetration of light into tissue enabling whole body, optical in vivo molecular imaging. Users must complete training and have current radiation safety certification.

LECO Pegasus GCxGC TOFMS, Facility:CLAS Goldwater Environmental Laboratory

Thumb Comprehensive two-dimensional chromatography (GCxGC) and a time-of-flight spectrometer (TOFMS) offers powerful detection in complex molecular mixtures. On-column cryofocusing of sample assists in detecting even very low volatility analytes. Orthogonal column phases paired with thermal modulation improves selectivity while dramatically raising signal-to-noise ratio (S/N) compared to one dimensional GC analysis. Utilizes Gerstel robotic sampling arm. SPME and Twister applications available. Please enquire for more information.

Leica CM1950 Cryostat, Facility:CLAS Bioimaging Facility - Keck Division

Thumb Leica CM1950 cryostat has a precise microtome object feed mechanism for high-quality sections, CryoZone™ system for optimal sectioning conditions and UVC disinfection and AgProtect™ for operator safety.

Leica RM2235 Microtome, Facility:CLAS Bioimaging Facility - Keck Division

Thumb Designed for manual routine paraffin embedded tissue sectioning.

Leica SP8 White Light Laser Confocal/Lightsheet , Facility:CLAS Bioimaging Facility - Keck Division

Thumb The Leica SP8 White Light Laser Confocal/Lightsheet inverted microscope system is designed for confocal live and fixed cell imaging. It is equipped with a white light tunable laser as well as Argon [488] and 405nm lasers. The system has a combination of PMT and HyD GaAsP detectors as well as a DIC bightfield detector. The system allows for standard confocal imaging with or without additional resonant scanning speed or can be switched into live cell mode by adding the Tokai Hit stage top incubation chamber and/or Light Sheet module and objectives.

Leica TCS SP5 AOBS Spectral Confocal System, Facility:CLAS Bioimaging Facility - Keck Division

Thumb The Leica SP5 has four laser lines (405, 488 [458, 476, 496,514], 561 and 633) and is capable of capturing 5 channels which includes a DIC bright field detector. The microscope is an upright DM6000 and has a motorized stage. Other features include; pixel resolution up to 8000x8000, AOTF laser modulation, 200 degree field rotation, standard objective set plus a 20x multi-emersion objective, electrophysiology software package and a Bioptics environmental chamber unit.

Map and information graphics preparation, Facility:Cartography and Information Graphics Lab

Preparation of publication-ready maps as well as information graphics -- charts, graphs and diagrams.

Matrix-Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF) Mass Spectrometer , Facility:Proteomics and Functional Genomics Core Facility

For routine protein characterization and identification, our Core Facility contains a Matrix-Assisted Laser Desorption Ionization TIme-of-Flight (MALDI-TOF) mass spectrometer (MS). Specifically, the unit is a Bruker Microflex LRF equipped with a 60 Hz laser and is capable of measuring masses of molecules ranging in size from several hundred to several hundred thousand Daltons. A comprehensive suite of software is available to assist in collecting, analyzing, interpreting, and presenting data.

Microbalances, Facility:CLAS Goldwater Environmental Laboratory

Thumb Capble of measuring to the microgram range. Typically used in the milligram and sub-milligram range. Two balances available for scheduling.

Microscale Thermophoresis (MST), Facility:Microscale Thermophoresis Facility

Thumb MicroScale Thermophoresis (MST) is an easy, fast and precise way to quantify biomolecular interactions. It measures the motion of molecules along microscopic temperature gradients and detects changes in their hydration shell, charge or size. When performing a MST experiment, a microscopic temperature gradient is induced by an infrared laser, and the directed movement of molecules is detected and quantified using either covalently attached dyes, or fluorescent fusion protein. MST detects interactions between any kind of biomolecules thus providing a large application range, from ions and small molecules to high molecular weight and multi-protein complexes. Current instrumentation is a Monolith NT.115 Series unit by NanoTemper Technologies. For access and assistance, please contact Dr. Julian Chen (JLChen@asu.edu, 480-965-3650) or Patrick Gleason (prgleaso@asu.edu, 480-965-1928)

Microwave Digestion System, Facility:CLAS Goldwater Environmental Laboratory

Thumb This system can be used to digest or extract a wide variety of solid and liquid materials. Common applications include soils, minerals and rocks, sediments, sludges, plant and animal tissues, water, dairy. Commonly used to prepare samples for metals and nutrient (total N, P, S) analysis. Will accomodate 40 samples of 10-20 mL volume (including digestion reagents). See CEM link for methods and application support.

Muffle Furnace - Fisher Isotemp 550, Facility:CLAS Goldwater Environmental Laboratory

Thumb Capable of ambient to 1250 degrees C.

NAPPA Protein Array Core, Facility:Biodesign Plasmid Repository and DNA Sequencing Facility

Thumb The NAPPA Protein Array Core uses a novel protein microarray technology, called Nucleic Acid-Programmable Protein Array (NAPPA), which replaces the complex process of spotting purified proteins with the simple process of spotting plasmid DNA. Our facility will offer high throughput DNA preparation, pre-set and custom protein array production, and array screening services. For move information, please contact the Core Manager.

Next-Generation Sequencing, Facility:Biodesign Plasmid Repository and DNA Sequencing Facility

Thumb The DNASU Sequencing facility offers Next Generation DNA Sequencing using the Illumina HiSeq 2000 platforms. This supports experimental applications such as high throughput DNA sequencing, genome wide association studies (GWAS), gene regulation analysis, SNP discovery and functional variation analysis, cytogenetic analysis, DNA-protein interaction analysis (ChIP-Seq), and sequencing-based methylation analysis.

Nikon Elipse TE300 Inverted Video Microscope, Facility:CLAS Bioimaging Facility - Keck Division

Thumb This imaging system consists of a Nikon Elipse TE300 inverted microscope and is outfitted with two cameras; a Hamamatsu Orca 3CCD and a Olympus DP26 color camera that allows real-time digital image capture. Both fluorescent and visible light (including both Phase and DIC contrast enhancement capabilities) are supported.

Nikon Microscope w/ Nanoject III, Facility:CLAS Bioimaging Facility - Keck Division

Thumb Nikon microscope equipped with Eppendorf micromanipulation controllers and a Drummond Scientific Nanojet III. Automated microprocessor controlled Microinjection Pipet delivering precise nanoliter volumes accurately. Remote control and non-rotating plunger eliminates vibration allowing precise injection minimizing possible cell rupture. Selectable Injection Volumes: from 2.3 nanoliters to 69.0 nanoliters in 16 increments.

Prairie Ultima IV 2-Photon Microscope, Facility:CLAS Bioimaging Facility - Keck Division

Thumb The Prairie Ultima IV 2P microscope comes equipped with an Ultima IV in vivo laser scanning microscope with an X-Y translation stage. The dual laser system includes the Mai Tai and Tsunami lasers capable of MPE imaging, oblation and uncaging.

Protein Separation and Preparation Equipment, Facility:Proteomics and Functional Genomics Core Facility

A variety of tools are available to assist with protein separation and preparation including a Bio-Rad LP protein purification system with fraction collector, benchtop centrifuges, standard and ultracentrifuges. A rotovap system is available to concentrate samples.

Sample Concentrator (Techne DB-3A), Facility:CLAS Goldwater Environmental Laboratory

Thumb Up to 24 samples can be concentrated with the use of temperatures up to 200˚C as well as an inert gas (or simply air). This instrument greatly accelerates the drying process and streamlines the scientific process.

Spectrophotometers, Facility:Proteomics and Functional Genomics Core Facility

The Core Facility contains a host of spectrophotometers and a plate reader to characterize and quantify proteins, nucleic acids, and other biological molecules. In addition, a nanodrop spectrophotometer is available.

Spectrophotometric Analysis, Facility:Lipid Soluble Nutrients Lab

use of liquid absorbance spectrophotometry to determine total concentration of vitamin A, vitamin E, or carotenoids

Technical Assistance, Facility:Lipid Soluble Nutrients Lab

Professional consultation and analytical services.

Thermal Cycler, Facility:Proteomics and Functional Genomics Core Facility

The Core Facility contains two thermal cyclers and an MJ Opticon real-time thermal cycler.

Total Organic Carbon / Nitrogen Analysis - Shimadzu TOC-V, Facility:CLAS Goldwater Environmental Laboratory

Thumb The Shimadzu TOC-VC/TN analyzes liquid samples for total carbon (TC), inorganic carbon (IC), total organic carbon (TOC), and non-purgeable organic carbon (NPOC). The TN unit allows for simultaneous TOC and Total Nitrogen (TN) analysis. A known amount of sample is introduced into the instrument and is either combusted (TC, TOC, NPOC) or acidified and purged (IC). Carbon content is measured as CO2 by non-dispersive infrared gas analysis. The TOC-VC combustion analyzer has a measuring range of 1mg C/L to 25,000mg C/L with reproducibility of 1.5% CV. It has a wide range of applications from ultra pure water to highly contaminated water. It is capable of multiple standard level and multiple constituent analyses in a single automated run.

VNMRS 800 MHz NMR, Facility:CLAS Magnetic Resonance Research Center

Thumb The VNMRS 800 MHz spectrometer is capable for liquids, solids, or MRI experiments. The instrument is ideally suited for the study of complex biomolecules.

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