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2D-HPLC, Facility:Center for Metabolic Biology Proteomics Lab

Michrom BioResources Paradigm MS4 micro 2-dimensional HPLC with autosampler.

96 Well Microplate Reader, Facility:CLAS DNA Laboratory

Thumb 96 Well Plate Reader: The Biotek HT1 Plate Reader scans multiple plate types in Absorbance, Fluorescence or Luminescence modes. Training is provided on the software and plate setup. The HT1 is equipped with gas control to allow anaerobic measurements or measurements under other environmental conditions. DNA and protein quantitation can also be done in a small volume 16 well plate. Reservations are taken for daily use.

Axon GenePix 4100A Microarray Scanner, Facility:Proteomics and Functional Genomics Core Facility

For microarray data acquisition and analysis, our Core Facility contains an Axon GenePix 4100A microarray scanner. GenePix software to facilitate data acquisition and analysis is also available.

BD FACS Aria Cell Sorter, Facility:Biodesign Fluorescence Assisted Cell Sorting Facility

This is a high-speed cell sorter. The instrument is equipped with three air-cooled lasers (488nm, 633nm and 407nm) and five fluorescent detectors, providing up to15 measurements (including 13 colors) of the cells. It offers high-speed cell sorting for isolation of rare populations of cells. In addition, the Automated Cell Deposition Unit (ACDU) allows cells sorted into multiwell plates or onto microscope slides. 1)Multicolor analysis of up to 15 parameters 2)Two- and four-way bulk sorting devices for a variety of tube sizes 3)Single cell sorting onto 6,12, 24 and 96 well plates 4) More applications can be explored.

BD FACS Calibur Cell Sorter & Analyzer, Facility:Biodesign Fluorescence Assisted Cell Sorting Facility

This is a single 488nm laser cell analyzer, which offers three color discrimination (520nm, 560 nm and 660 nm). The major application of this instrument: 1)Examination of cell surface/cytoplasmic markers (up to 3 colors), 2) GFP/RFP identification, 3)DNA synthesis and cell division assessment 4)Apoptosis and necrosis analysis, 5) Multi-cytokine array profiling

BI-2000 SPR, Facility:Biodesign Biosurface Chemistry Facility

Thumb BI-2000 has a unique detection technology that delivers high sensitivity (< 10-4 degrees), thusenables researchers to measure a binding constant down to a few pM-1. The system was developed to meet many application requirements with various interchangeable analysis modules such as the Flow Injection Analysis Module for DNA sequencing, protein/protein interaction, ligand/receptor recognition, and drug development applications. The Electrochemcial and EC-DualFlow™ Analysis Modules for electrochemical SPR.Applications:Flow Injection Analysis Module for two channel flow injection SPR researchElectrochemical Analysis Module for electrochemical (EC) SPR research.Materials Avaliable:SPR Gold Sensor Chips (16mmx16mm) or (22mmx22mm) 49nm sputter coated (Au/Cr)

Biacore A100, Facility:Biodesign CIM Proteomics Facility

Biacore® A100 is a protein interaction analysis system for kinetic, affinity, concentration and specificity data. It is designed for applications requiring high sample throughput using small panels of proteins, but can also be used for detailed characterisation studies, enabling integration into multiple phases of project pipelines. Confident selection and optimization of lead compounds during drug discovery – label-free screening and characterization Reduce costs in biotherapeutic development – early kinetic screening of hybridomas for mAb selection Optimize safety and efficacy – define serum antibody responses in immunogenicity studies and immunotherapeutic development Perform large scale functionality studies – high quality, high information-content interaction studies in proteomics

Biacore Flexchip, Facility:Biodesign CIM Proteomics Facility

The Biacore Flexchip is designed to allow users to compare and rank hundreds of interactions in real time, in terms of kinetics, affinity or specificity without the use of labels; map interaction networks and identify potential therapeutic targets; screen and select protein therapeutics; investigate the significance of variations in glycosylation on protein function; screen or profile binders against whole cell targets.

Biacore T100, Facility:Biodesign CIDV Core Facility

Biacore T100 is a system for comprehensive protein interaction analysis from early research, to drug discovery and development and on to QC. The system is supported by state of the art software for assay development, analysis, data evaluation and interpretation. Software wizards ensure ease of use. Alternatively, methods may be developed or customized as required. In addition to providing detailed information on kinetics and affinity, software support allows interactions to be thermodynamically characterized. Elucidate disease mechanisms by characterizing native or recombinant protein interactions Select the best antibodies as research tools, assay components or therapeutics by fully defining their interaction behavior Define potential drug targets and diagnostic markers Characterize protein: LMW compound interactions in drug discovery Select protein therapeutic candidates according to their on/off rates Detect and characterize immune responses during preclinical and clinical development - read more Characterize protein therapeutics and implement QC methods Recover interaction partners for characterization by mass spectrometry

Bio-Rad Versadoc 400 Gel Imaging System, Facility:Proteomics and Functional Genomics Core Facility

Capable of imagaging both nucleic acid and protein gels.

Bioanalyzer (Agilent 2100), Facility:CLAS DNA Laboratory

Thumb Bioanalyzer: The Agilent 2100 Bioanalyzer is a capillary electrophoresis unit capable of separating RNA, DNA or protein in lieu of gel electrophoresis. You may submit samples as a service request or you can be trained how to use the instrument. We routinely run RNA and DNA samples only, so please contact us before planning to submit any other samples. Chip types usually on hand are DNA1000, High Sensitivity DNA, RNA6000 Nano and RNA6000 Pico. Reservations are taken for daily use.

Bruker Avance III HD 600 MHz NMR, Facility:CLAS Magnetic Resonance Research Center

Thumb This Bruker 600 MHz NMR is equipped with a 5 mmTriple Resonance Prodigy Cryoprobe. CPP TCI 600 H&F-C/N-D-5-Z. It is ideally suited for biological and protein structure determination. Additionally protein and bimolecular dynamics in solution can be studied with this instrument.

Bruker Avance III HD 850 MHz NMR, Facility:CLAS Magnetic Resonance Research Center

Thumb The Bruker 850 MHz spectrometer is equipped with a 5 mm H&F-C/N-D TCI Cryoprobe, a 1.7 mm H-C/N-D TCI Cryoprobe, and 1.3 mm Ultrafast HX MAS probe. This instrument is equipped for liquids and solids. The instrument is ideally suited for the study of complex biomolecules.

Bruker Biospin AVANCETM III 400 NMR, Facility:Proteomics and Functional Genomics Core Facility

The Bruker Biospin AVANCETM III 400 Spectrometer System utilizes a superconducting magnet system with 5.4 cm bore (operation field at 9.395 Tesla). The unit is equipped with a sample transport system that enables high flow rates for VT gas. Bruker TopSpin software is used for data acquisition and processing.

Centrifuge (Allegra X-14 Benchtop) , Facility:CLAS Goldwater Environmental Laboratory

Thumb Centrifuge has both swinging bucket rotor (max. 4300 rpm) and fixed angle rotor (max. 10,200 rpm) capabilities. Rotors have adaptors for both 15 ml and 50 ml conical vials. Centrifuge has capability for both timed or user programed options.

DNA Sequencing - High Throughput, Facility:Biodesign Plasmid Repository and DNA Sequencing Facility

Thumb The DNA sequencing services at Center for Personalized Diagnostics (CPD) provides investigators access to automated DNA sequencing on a chargeback basis. Sequencing is available for purified DNA and glycerol stocks. The facility specializes in large (more than 48 samples) projects. Turn-around-time for sequencing reactions less than 48 samples will be dependent on sample queue. We will make every effort to turn these around as quickly as possible. Sequence data is posted on our secure website for users to download and analyze. The facility uses Big Dye V3.1 chemistry with samples processed using an Applied Biosystems 3730XL Sequence Analysis Instrument.

DNA Sequencing and Fragment Analysis, Facility:CLAS DNA Laboratory

Thumb DNA sequencing is run as a service. Laboratories may submit samples along with the sequencing request form. Sequencing reactions are usually run in our lab. However, we will run, at reduced cost, sequencing reactions — run in other laboratories. We will purify the reactions before running. We maintain a stock of common plasmid primers accessible on the sequencing form. We use an Applied Biosystems 3730 capillary sequencer. Read lengths are routinely 800–900bp. Turnaround time is 24–48 hours. We can also perform plasmid or pcr purifications in conjunction with sequencing. Fragment Analysis, which includes microsatellites, AFLP, or STR applications, are run on the 3730 sequencer. Dye-labeled samples are normally generated in respective laboratories and delivered to our lab for analysis. Contact us if you have questions on dye compatibility and before submitting samples.

DNA Size Fractionation, Facility:CLAS DNA Laboratory

Thumb DNA Size Fractionation: Preparative DNA size fractionation can be run on a Sage Scientific Pippen Prep. Recovered samples are suitable for Next-Gen sequencing. There can be up to four samples per run.

DNASU Plasmid Repository - Collection Clones, Facility:Biodesign Plasmid Repository and DNA Sequencing Facility

Thumb A collection of 191,000 plasmids with genes from more than 300 organisms that includes clones created in Dr. LaBaer's laboratory, human and mouse genes from the ORFeome collaboration, plasmids created by researchers in the NIGMS-funded Protein Structure Initiative (PSI) after we became the PSI Material Repository (PSI-MR; http://psimr.asu.edu) in 2006, and various plasmids from outside researchers. We distribute special collections of clones such as sets of human kinases, breast cancer-related genes, and transcription factors as well as whole genome collections for a variety of organisms. Distribution of these plasmids to researchers at ASU and around the world is through http://dnasu.org. Browse all collection here.

DNASU Plasmid Repository - Individual Clones, Facility:Biodesign Plasmid Repository and DNA Sequencing Facility

Thumb A collection of 191,000 plasmids with genes from more than 600 organisms that includes clones created in Dr. LaBaer's laboratory, human and mouse genes from the ORFeome collaboration, plasmids created by researchers in the NIGMS-funded Protein Structure Initiative (PSI) after we became the PSI Material Repository (PSI-MR; http://psimr.asu.edu) in 2006, and various plasmids from outside researchers . Distribution of these plasmids to researchers at ASU and around the world is through http://dnasu.org. In addition to plasmid distribution, we also accept deposits of both large and small plasmid collections from researchers and distribute their clones to researchers worldwide. For more information about either of these services, please contact us.

Discrete Analyzer - Seal AQ2, Facility:CLAS Goldwater Environmental Laboratory

Thumb The AQ2 system is a flexible automated analyzer that uses the principle of Discrete Analysis where each test occurs in a separate or discrete reaction vessel. The AQ2 is ideal for measuring dissolved nutrients and other constituents in aqueous samples, extracts, and digestions. It is most useful when many and varied tests are needed on different samples and/or individual results are needed immediately. The sample and reagents are incubated in the reaction wells for a pre-programmed time. A single aliquot is then transferred into a 10mm 100% optical glass cuvette. The absorbance is read on the stationary reactant to ensure the best possible signal to noise ratio. Methods: Alkalinity Ammonia Chloride Cyanides Nitrate/Nitrite Nitrite Nitrogen, Total (Persulfate) Phenolics Phosphate, Ortho Phosphorus, Total (Persulfate) Silicate Sulfate

Evos FL Auto Live Cell Imaging System, Facility:CLAS Bioimaging Facility - Keck Division

Thumb The Evos inverted microscope system is equipped with an onstage incubator for environmental control and can accommodate multi-well plates and standard round culture dishes. The fluorescent/widefield system is capable of z stage movement and tiling. Phase corrected objectives include 4xPH, 10xPH, 20xL PH, 40xL PH as well as a 60x 1.42NA oil lens for higher resolution imaging. Four LED light cubes are available to cover the standard DAPI, GFP, RFP and Cy5 wavelengths.

HP1100 HPLC-ELSD, Facility:Biodesign Biosurface Chemistry Facility

Thumb HP1100 Preperative/Analytical HPLC and PL-ELS-2100 Ice Evaporative Light Scattering Detector (ELSD).  Instrument can separate, identify, and quantify compounds using Reverse Phase or Normal Phase methods. Also analyze non-UV absorbing compounds with PL-ELS-2100 Ice. Columns Avaliable: (Various Companies)

HPLC Analysis, Facility:Lipid Soluble Nutrients Lab

use of reverse-phase high performance liquid chromatography to determine types and amounts of carotenoids in plant or animal tissue

KODAK Image Station In-Vivo FX, Facility:CLAS Bioimaging Facility - Keck Division

Thumb The In-Vivo FX offers quantitative imaging of luminescent, radioisotopic and fluorescent labeled biomolecules in combination with radiographic imaging, providing a versatile in vivo imaging system for small animals. It also has the ability to select and apply longer excitation wavelengths (green to near-IR) which improves the penetration of light into tissue enabling whole body, optical in vivo molecular imaging. Users must complete training and have current radiation safety certification.

LECO Pegasus GCxGC TOFMS, Facility:CLAS Goldwater Environmental Laboratory

Thumb Comprehensive two-dimensional chromatography (GCxGC) and a time-of-flight spectrometer (TOFMS) offers powerful detection in complex molecular mixtures. On-column cryofocusing of sample assists in detecting even very low volatility analytes. Orthogonal column phases paired with thermal modulation improves selectivity while dramatically raising signal-to-noise ratio (S/N) compared to one dimensional GC analysis. Utilizes Gerstel robotic sampling arm. SPME and Twister applications available. Please enquire for more information.

Leica CM1950 Cryostat, Facility:CLAS Bioimaging Facility - Keck Division

Thumb Leica CM1950 cryostat has a precise microtome object feed mechanism for high-quality sections, CryoZone™ system for optimal sectioning conditions and UVC disinfection and AgProtect™ for operator safety.

Leica SP8 White Light Laser Confocal/Lightsheet , Facility:CLAS Bioimaging Facility - Keck Division

Thumb The Leica SP8 White Light Laser Confocal/Lightsheet inverted microscope system is designed for confocal live and fixed cell imaging. It is equipped with a white light tunable laser as well as Argon [488] and 405nm lasers. The system has a combination of PMT and HyD GaAsP detectors as well as a DIC bightfield detector. The system allows for standard confocal imaging with or without additional resonant scanning speed or can be switched into live cell mode by adding the Tokai Hit stage top incubation chamber and/or Light Sheet module and objectives.

Leica TCS SP5 AOBS Spectral Confocal System, Facility:CLAS Bioimaging Facility - Keck Division

Thumb The Leica SP5 has four laser lines (405, 488 [458, 476, 496,514], 561 and 633) and is capable of capturing 5 channels which includes a DIC bright field detector. The microscope is an upright DM6000 and has a motorized stage. Other features include; pixel resolution up to 8000x8000, AOTF laser modulation, 200 degree field rotation, standard objective set plus a 20x multi-emersion objective, electrophysiology software package and a Bioptics environmental chamber unit.

Matrix-Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF) Mass Spectrometer , Facility:Proteomics and Functional Genomics Core Facility

For routine protein characterization and identification, our Core Facility contains a Matrix-Assisted Laser Desorption Ionization TIme-of-Flight (MALDI-TOF) mass spectrometer (MS). Specifically, the unit is a Bruker Microflex LRF equipped with a 60 Hz laser and is capable of measuring masses of molecules ranging in size from several hundred to several hundred thousand Daltons. A comprehensive suite of software is available to assist in collecting, analyzing, interpreting, and presenting data.

Microarray Analysis, Facility:CLAS DNA Laboratory

Thumb Microarray analysis: Slides can be scanned using an Agilent scanner. We do not manufacture microarrays. We do have the equipment to complete all other aspects of microarray analysis. Files generated from the scanner are processed using either Agilent’s Feature Extraction or Axon’s GenePix Pro 6.0 software.. Normally we instruct users on the techniques and software. However, we will provide microarray hybridization and analysis to laboratories that provide us the RNA. Contact us for more information. Any RNA submitted for microarray analysis will be checked on the bioanalyzer for integrity before beginning the labeling reactions.

Microscale Thermophoresis (MST), Facility:Microscale Thermophoresis Facility

Thumb MicroScale Thermophoresis (MST) is an easy, fast and precise way to quantify biomolecular interactions. It measures the motion of molecules along microscopic temperature gradients and detects changes in their hydration shell, charge or size. When performing a MST experiment, a microscopic temperature gradient is induced by an infrared laser, and the directed movement of molecules is detected and quantified using either covalently attached dyes, or fluorescent fusion protein. MST detects interactions between any kind of biomolecules thus providing a large application range, from ions and small molecules to high molecular weight and multi-protein complexes. Current instrumentation is a Monolith NT.115 Series unit by NanoTemper Technologies. For access and assistance, please contact Dr. Julian Chen (JLChen@asu.edu, 480-965-3650) or Patrick Gleason (prgleaso@asu.edu, 480-965-1928)

NAPPA Protein Array Core, Facility:Biodesign Plasmid Repository and DNA Sequencing Facility

Thumb The NAPPA Protein Array Core uses a novel protein microarray technology, called Nucleic Acid-Programmable Protein Array (NAPPA), which replaces the complex process of spotting purified proteins with the simple process of spotting plasmid DNA. Our facility will offer high throughput DNA preparation, pre-set and custom protein array production, and array screening services. For move information, please contact the Core Manager.

Next-Generation Sequencing, Facility:Biodesign Plasmid Repository and DNA Sequencing Facility

Thumb The DNASU Sequencing facility offers Next Generation DNA Sequencing using the Illumina HiSeq 2000 platforms. This supports experimental applications such as high throughput DNA sequencing, genome wide association studies (GWAS), gene regulation analysis, SNP discovery and functional variation analysis, cytogenetic analysis, DNA-protein interaction analysis (ChIP-Seq), and sequencing-based methylation analysis.

Nikon Elipse TE300 Inverted Video Microscope, Facility:CLAS Bioimaging Facility - Keck Division

Thumb This imaging system consists of a Nikon Elipse TE300 inverted microscope and is outfitted with two cameras; a Hamamatsu Orca 3CCD and a Olympus DP26 color camera that allows real-time digital image capture. Both fluorescent and visible light (including both Phase and DIC contrast enhancement capabilities) are supported.

Nikon Microscope w/ Nanoject III, Facility:CLAS Bioimaging Facility - Keck Division

Thumb Nikon microscope equipped with Eppendorf micromanipulation controllers and a Drummond Scientific Nanojet III. Automated microprocessor controlled Microinjection Pipet delivering precise nanoliter volumes accurately. Remote control and non-rotating plunger eliminates vibration allowing precise injection minimizing possible cell rupture. Selectable Injection Volumes: from 2.3 nanoliters to 69.0 nanoliters in 16 increments.

Peptide Synthesis, 21 to 36-mer, Facility:SOLS Peptide Synthesis Lab

10 mg of 90% pure peptide using a Protein Technologies Symphony 12-Channel Peptide Synthesizer.

Peptide Synthesis, up to 20-mer, Facility:SOLS Peptide Synthesis Lab

10 mg of 90% pure peptide using a Protein Technologies Symphony 12-Channel Peptide Synthesizer.

Prairie Ultima IV 2-Photon Microscope, Facility:CLAS Bioimaging Facility - Keck Division

Thumb The Prairie Ultima IV 2P microscope comes equipped with an Ultima IV in vivo laser scanning microscope with an X-Y translation stage. The dual laser system includes the Mai Tai and Tsunami lasers capable of MPE imaging, oblation and uncaging.

Protein Separation and Preparation Equipment, Facility:Proteomics and Functional Genomics Core Facility

A variety of tools are available to assist with protein separation and preparation including a Bio-Rad LP protein purification system with fraction collector, benchtop centrifuges, standard and ultracentrifuges. A rotovap system is available to concentrate samples.

Proteomelab PA 801, Facility:Biodesign CIDV Core Facility

Resolves and quantifies proteins by their isoelectric point and molecular weight, generates high-resolution peptide maps and carbohydrate profiles and provides front-end separation to mass spectrometry.

Real-Time PCR (ABI 7900), Facility:CLAS DNA Laboratory

Thumb Real time PCR in the DNA lab is run on an ABI7900HT thermocycler using a 384 well format. Samples are usually set up in respective laboratories. We provide training on the software and help in experimental setup, if required. Primers and probes can be designed using ABI Primer Express software available in the lab. Reservations are taken for three daily time slots.

Sample Concentrator (Techne DB-3A), Facility:CLAS Goldwater Environmental Laboratory

Thumb Up to 24 samples can be concentrated with the use of temperatures up to 200˚C as well as an inert gas (or simply air). This instrument greatly accelerates the drying process and streamlines the scientific process.

Spectrophotometers, Facility:Proteomics and Functional Genomics Core Facility

The Core Facility contains a host of spectrophotometers and a plate reader to characterize and quantify proteins, nucleic acids, and other biological molecules. In addition, a nanodrop spectrophotometer is available.

Thermal Cycler, Facility:Proteomics and Functional Genomics Core Facility

The Core Facility contains two thermal cyclers and an MJ Opticon real-time thermal cycler.

UVP EpiChemi3 Darkroom Gel Imaging/Scanner, Facility:Biodesign Biosurface Chemistry Facility

Thumb Chemilluminescent scanner. Sample Applications include SYBR Green, Ethidium Bromide, Coomassie Blue, SYBR Gold andn CY3. 

VNMRS 800 24 Hours, Facility:CLAS Magnetic Resonance Research Center

Thumb 800 MHz Ultra-Shield Varian NMR * Organic & Inorganic Molecular Structure & Dynamics * Biomolecular and Protein Structure & Dynamics * Biopolymers and Materials * 3D Micro-imaging and Molecular Diffusion

VNMRS 800 MHz NMR, Facility:CLAS Magnetic Resonance Research Center

Thumb The VNMRS 800 MHz spectrometer is capable for liquids, solids, or MRI experiments. The instrument is ideally suited for the study of complex biomolecules.

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